Journal: Cytotechnology

Article Title: A comparative study of colorimetric cell proliferation assays in immune cells

doi: 10.1007/s10616-015-9909-2

Figure Lengend Snippet: Cell number range for MTT, WST-1, and resazurin. a A serial number of mouse whole spleen cells were seeded into a 96-well plate in duplicates or triplicates, and they were incubated without stimulation in the absence of any reagent for the first 1 h at 37 °C. Three different reagents were then individually added, followed by incubation for the last 4 h at 37 °C. Each absorbance value was assessed by a plate reader. b The figures in a were normalized to the respective minimal values and combined into the same graph to compare their fold change. These are representative of at least three experiments with similar results

Article Snippet: Antibodies and reagents MTT was purchased from Dojindo (Kumamoto, Japan) and WST-1 from Wako (Osaka, Japan).

Techniques: Incubation

Journal: Cytotechnology

Article Title: A comparative study of colorimetric cell proliferation assays in immune cells

doi: 10.1007/s10616-015-9909-2

Figure Lengend Snippet: TCR signal does not substantially raise MTT activity before cell division. Murine whole spleen cells were stained with or without CFSE fluorescent dye and stimulated with a serially diluted amount of anti-CD3 and -CD28 Abs for 16 h. a The cells were subjected to flow cytometry for FSC/SSC analysis and CFSE fluorescence intensity dilution assay. FSC and SSC reflect cell size and intracellular granular structures, respectively. b The same cells were given MTT, WST-1, or resazurin for the last 4 h of 16 h and subjected to the absorbance assays by a plate reader. These are representative of five experiments with similar results. c The FSC result shown in a is expressed as a graph

Article Snippet: Antibodies and reagents MTT was purchased from Dojindo (Kumamoto, Japan) and WST-1 from Wako (Osaka, Japan).

Techniques: Activity Assay, Staining, Flow Cytometry, Fluorescence, Dilution Assay

Journal: Cytotechnology

Article Title: A comparative study of colorimetric cell proliferation assays in immune cells

doi: 10.1007/s10616-015-9909-2

Figure Lengend Snippet: Dose-response curve of MTT, WST-1, and resazurin. a Mouse spleen cells were examined for the dose–response curve of MTT, WST-1, and resazurin against a serially diluted amount of anti-CD3 Ab (0.01, 0.1, and 1.0 μg/mL) in the constant presence of anti-CD28 Ab costimulation (0.25 μg/mL) for 2 days. b The figures in a were normalized to the respective minimal values and combined into the same graph to compare their fold change. These are representative of six experiments with similar results

Article Snippet: Antibodies and reagents MTT was purchased from Dojindo (Kumamoto, Japan) and WST-1 from Wako (Osaka, Japan).

Techniques:

Journal: Cytotechnology

Article Title: A comparative study of colorimetric cell proliferation assays in immune cells

doi: 10.1007/s10616-015-9909-2

Figure Lengend Snippet: Kinetics of responses to stimulation. Mouse whole spleen cells were examined for the kinetic response of MTT, WST-1, and resazurin to stimulation using anti-CD3 Ab (0.5 μg/mL) in the presence of anti-CD28 Ab (0.25 μg/mL) for 1–4 days. These are representative of two experiments with similar results

Article Snippet: Antibodies and reagents MTT was purchased from Dojindo (Kumamoto, Japan) and WST-1 from Wako (Osaka, Japan).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Pravastatin Attenuates Acute Radiation-Induced Enteropathy and Improves Epithelial Cell Function

doi: 10.3389/fphar.2018.01215

Figure Lengend Snippet: Pravastatin enhances epithelial cell proliferation after radiation-induced intestinal damage. (A) WST-1 assays and (B) SA β-gal staining of control (Con), irradiated (IR), and pravastatin-treated IR (IR + Prava) InEpC cells. n = 3 per group. (C) Counting of Ki-67-positive cell in the intestine of Con, IR, and IR + Prava mice. Bar = 200 μm. Data are presented as the mean ± standard error of the mean; n = 5 mice per group. ∗ P < 0.05 compared to the control; # P < 0.05 compared to the IR group.

Article Snippet: Cell proliferation was evaluated using a colorimetric method based on WST-1 (CellVia, Abfrontier, Seoul, South Korea).

Techniques: Staining, Irradiation